Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

Screening Lipid Structures Using Stable Isotope Labeling

August 23, 2024

Traçage de novo des lipides à l’aide du marquage des isotopes stables LC-TIMS-TOF MS/MS

00:47 min

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Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

02:50 min

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LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

03:39 min

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Katherine D. Castro, Lilian Valadares Tose, Matthew Willetts, Melvin A. Park, Marcela Nouzova, Fernando G. Noriega⁵, Francisco Fernandez-Lima⁶

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

Summary

Voici une méthode de criblage des structures lipidiques par marquage d’isotopes stables en utilisant leur temps de rétention, leur mobilité et leur fragmentation.

Screening Lipid Structures Using Stable Isotope Labeling

Traçage de novo des lipides à l’aide du marquage des isotopes stables LC-TIMS-TOF MS/MS

Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

Summary

Article