Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

Screening Lipid Structures Using Stable Isotope Labeling

August 23, 2024

Rastreamento de lipídios de novo usando marcação de isótopos estáveis LC-TIMS-TOF MS/MS

00:47 min

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Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

02:50 min

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LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

03:39 min

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Katherine D. Castro, Lilian Valadares Tose, Matthew Willetts, Melvin A. Park, Marcela Nouzova, Fernando G. Noriega⁵, Francisco Fernandez-Lima⁶

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

Summary

Aqui é apresentado um método para triagem de estruturas lipídicas por meio da marcação de isótopos estáveis, usando seu tempo de retenção, mobilidade e fragmentação.

Screening Lipid Structures Using Stable Isotope Labeling

Rastreamento de lipídios de novo usando marcação de isótopos estáveis LC-TIMS-TOF MS/MS

Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

LC-TIMS-TOF MS/MS Data Analysis for Screening Lipids with Stable Isotope Labeling

Summary

Article