Crypt Isolation from Murine Small Intestine - JoVE

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Isolement de la crypte de l’intestin grêle murin

Published: June 02, 2023

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Yuta Takase, Kazuto Fujishima³, Toshio Takahashi

¹Suntory Foundation for Life Sciences,Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, ³Faculty of Medicine,Osaka Medical and Pharmaceutical University

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Keywords: Crypt Isolation Murine Small Intestine PBS Antibiotics EDTA Extracellular Matrix ECM DMEM Sorbitol Cell Strainer Cell Culture Centrifugation Microscopy

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Crypt Isolation from Murine Small Intestine. J. Vis. Exp. (Pending Publication), e200007, doi:10.3791/200007 (2023).

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