This video describes a method to mechanically remove the chorion and vitelline membranes from previously fixed mature Drosophila oocytes. The featured protocol shows a detailed demonstration of the procedure yielding oocytes compatible with fluorescent in situ hybridization assays.
Protocol
This protocol is an excerpt from Perkins and Bickel, Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes, J. Vis. Exp. (2017). 1. Removal of Chorions and Vitelline Membranes NOTE: See Figure 1 for tools needed. Separate late stage oocytes<…
Representative Results
Figure 1: Cytology tools. Tools used in protocol: (1) pair of forceps (#5 Dumont); (2) tungsten needle; (3) deep well dish with cover; (4) shallow glass dissecting dish; (5) pulled Pasteur pipette. Please click here to view a larger version of this figure. <img alt="Figure 2" class="xfigimg" src="/…
Chorion and Vitelline Membrane Mechanical Removal: A Method to Prepare Drosophila Oocytes for Direct Observation. J. Vis. Exp. (Pending Publication), e20124, doi: (2023).