Seeding Organ Chips with Human Uterine Fibroblasts (HUFs) for Studying Living Vaginal Microbiome

doi:10.3791/201539

JoVE Journal > Bioengineering

生物工程

Seeding Organ Chips with Human Uterine Fibroblasts (HUFs) for Studying Living Vaginal Microbiome

Published: February 16, 2024

doi:

10.3791/201539

Aakanksha Gulati, Alicia Jorgenson, Abidemi Junaid, Donald E. Ingber^2,3

¹Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Vascular Biology Program and Department of Surgery,Boston Children’s Hospital and Harvard Medical School, ³Harvard John A. Paulson School of Engineering and Applied Sciences

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Organ Chips Human Uterine Fibroblasts (HUFs)Vaginal Microbiome Cell Culture Cell Seeding Microfluidic Devices Fibroblast Medium Vaginal Epithelial Medium Cell Counting Trypsin EDTA Trypan Blue Centrifugation Cell Attachment Incubation

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Seeding Organ Chips with Human Uterine Fibroblasts (HUFs) for Studying Living Vaginal Microbiome. J. Vis. Exp. (Pending Publication), e201539, doi:10.3791/201539 (2024).

Seeding Organ Chips with Human Uterine Fibroblasts (HUFs) for Studying Living Vaginal Microbiome

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