Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model
Published: April 30, 2023
Abstract
Source: Krishnan, V. et al. Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose. J. Vis. Exp. (2015)
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent metastasis.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intraperitoneal Injection of Cells NOTE: In our experiments, mice are handled in a laminar airflow or biosafety cabinet within our barrier facility in order to limit the risk of pathogen exposure. In order to demonstrate this technique clearly, the procedures in the accompanying video were condu…
Disclosures
The authors have nothing to disclose.
Materials
| DMEM | Corning | 10-013-CV | |
| PBS | Corning | 21-040-CV | Without calcium and Magnesium |
| 26 gauge needle | BD | 329652 |
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Cite This Article
Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model. J. Vis. Exp. (Pending Publication), e20377, doi: (2023).