Extracting Prostate from Mouse Urogenital System: A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse

Abstract

Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. Vis. Exp. (2018)

In this video, we describe the procedure for the dissection of the urogenital system or UGS from a male mouse model. This UGS is further dissected ex vivo to isolate the prostate gland and study its role in prostate cancer development.

Protocol

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. The Urogenital System (UGS) Dissection NOTE: The schematic is presented in Figure 1. Euthanize a 3-month-old male C57BL/6 mouse using the CO2 inhalational euthanasia method or another approved technique. NOTE: Mice between the age…

Representative Results

Figure 1:  Schematic flowchart demonstrating dissection of the mouse urogenital system and isolation of the prostate. Flow chart for the protocol steps described in sections 1 Figure 2: Dissection of the mouse UGS….

Materials

Mouse surgical instruments (Mouse Dissecting kit)	World Precision Instruments	MOUSEKIT
Dissection microscope
Dissection medium (DMEM + 10%FBS)	Thermofisher Scientific	11965-084
Fetal Bovine Serum	Thermofisher Scientific	10438018