Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma

Abstract

Source: Moret, R. et al. Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis. J. Vis. Exp. (2019)

This video describes the procedure to generate an experimental xenograft murine model, which can be used for studying urothelial cell carcinoma progression.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. UCC Mouse Model Preparation of mice for procedure Obtain 6- to 8-week old female NOD/SCID mouse. Shave the lower backs of the mouse using hair removal cream. Anesthetize the mouse in an induction chamber with isoflurane (2.5% in 100% oxygen, 1 L/min). Once sedated, place the mouse in the supine po…

Representative Results

Figure 1: Orthotopic UCC mouse models. (Aa) An angiocatheter was inserted into the bladder of a female NOD/SCID mouse and an electrocautery shock was applied to the bladder wall via a guidewire. (Ab) Luciferase tagged UCC tumor cells, BlCaPt15 (2 x 104 cells), or BlCaPt37 (5 x 105 cells) with the addition of 3 x 105 </sup…

Materials

Hair removal cream	Church & Dwight Co., Inc	1(800)248-8820
Isoflurane	Henry Schein Animal Health	108333
6-8 week old NOD/SCID mice (female)	Jackson Lab	1303
Forceps	Symmetry Surgical Inc	06-0011
Electrosurgical generator	ValleyLab	FORCE1C20
Isoflurane induction chamber	Perkin Elmer	119038