5 ml polystyrene round-bottom tube |
Falcon |
352054 |
Tubes for DS containing enzymes used in digestion steps |
Amphotericin-B |
Fisher |
BP928-250 |
Used for patch/cell perforation |
Amphotericin-B |
European Pharmacopoeia Reference Standards |
5 |
Used for patch/cell perforation |
Amphotericin-B |
Sigma-Aldrich |
A9528-100MG |
Used for patch/cell perforation |
Analog vortex mixer |
VWR |
58816-121 |
|
Aspartic acid |
Sigma-Aldrich |
A9006 |
Intracellular pipette solution |
Bovine serum albumin |
Sigma-Aldrich |
A7906 |
DS |
CaCl2 |
Sigma-Aldrich |
C1016 |
Extracellular solution and DS |
Capillary Glass |
Sutter |
BF150-110-7.5 |
Capillary for preparation of pulled patch electrodes |
Cesium hydroxide hydrate |
Sigma-Aldrich |
C8518 |
Intracellular pipette solution |
Clampex ver. 10 software includes data acqusition (Clampex) and analysis (Clampfit) programs |
Axon Instruments/ Molecular Devices |
pCLAMP-10 |
Commerical software and part of patch-clamp rig setup |
CsCl |
Sigma-Aldrich |
203025 |
Extracellular and intracellular solutions |
Dental wax |
Miltex Dental Wax Technologies, Inc. |
18058351 |
|
Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
D2650 |
Solvent |
EGTA |
Sigma-Aldrich |
E3889 |
Ca2+ chelator, used in intracellular pipette solution |
Flaming/Brown micropipette puller |
Sutter |
P-97 |
Required to pull electrodes with very fine tips |
Floating foam tube rack/holder |
VWR Scientific |
82017-634 |
Used for holding tubes with enzymes for temperature control |
Glucose |
Sigma |
G8270 |
|
Glutamic acid (Na salt) |
Sigma-Aldrich |
G1626 |
DS |
HEPES |
Sigma-Aldrich |
H3375 |
pH Buffer |
KCl |
Fisher Scientific |
BP366-1 |
Extracellular solution |
Low Noise Data Acquisition System |
Axon Instruments/ Molecular Devices |
Digidata 1440A |
Part of patch-clamp rig setup |
Magnetic stirrer |
VWR |
01-442-684 |
|
MgCl2 (hexahydrate) |
Sigma-Aldrich |
M2670 |
Extracellular and intracellular solutions |
MicroForge |
Narishige |
MF-830 |
Used for fire-polishing electrodes |
NaCl |
Sigma-Aldrich |
S7653 |
Extracellular and intracellular solutions |
NaOH |
Sigma-Aldrich |
S8045 |
|
Nifedipine |
Sigma-Aldrich |
N7634 |
L-type voltage-gated Ca2+ channel blocker |
Nikon inverted microscope, TS100 with T1-SM stage with 5x, 10x, 20x, and 40x objectives |
Nikon |
Discontinued |
Part of Patch-clamp rig setup |
Non-metalic syringe needle, MicroFil |
WPI |
MF-34G-5 |
Filling of intracellular pipette solution |
Pasteur pipette |
FisherBrand |
13-678-20A |
Tips are broken off and fire-polished and used for titration of enzymatically treated tissues to release single DSM cells from pieces |
Patch-clamp amplifier |
Axon Instruments/ Molecular Devices |
Axon Axopatch 200B |
Part of patch-clamp rig setup |
PC computer |
DELL |
Custom configuration |
Part of patch-clamp rig setup |
pH Meter |
Aspera Instruments |
PH700 |
|
Polyethylene tubing |
Intramedic |
427-436 |
Tubing for superfusion of extracellular bath connected to glass-bottom recording chamber |
Tetraethylammonium chloride |
Sigma-Aldrich |
T2265 |
Ion channel blocker of Kv and BK channels added to the extracellular bath solution |
Weighting scale |
Mettler Toledo |
XS64 |
|
ZeissAxiovert 40C inverted microscope with 10x and 40x objectives |
Carl-Zeiss |
Discontinued |
Part of patch-clamp rig setup |