In this video, we show a procedure for simultaneous recording of video-EEG-ECG-capnography-oximetry data generated by inducing seizures in a rabbit model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Photic stimulation experiment Place a light source with a circular reflector 30 cm in front of the rabbit at eye level, with the flash intensity set to the maximum (16 candela). The light source is indicated by a white dot in Figure 1E. NOTE: A dimly lit room should be used to elicit the pho…
Representative Results
Figure 1: Rabbit connected to equipment. (A) Location of ECG electrodes, Left Arm is indicated by a yellow dot. Right Arm is indicated by a white dot. Left Leg is indicated by a red dot. Ground anterior to the right leg is indicated by a green dot. (B) Rabbit in restrainer with ECG and EEG electrodes attached. (C) Juvenile rabbit in a restrainer …
Disclosures
The authors have nothing to disclose.
Materials
Computer
Dell
Optiplex 5040
Acquisition computer
ECG Electrode
RhythmLink
RLSND116-2.5
13mm 35-degree bent (0.4 mm diameter) subdermal pin electrodes
Intermittent Photic Stimulation in Rabbit Model: A Technique to Induce Epileptic Seizures and Simultaneously Record Multisystem Parameters. J. Vis. Exp. (Pending Publication), e20824, doi: (2023).