Materials
| Name | Company | Catalog Number | Comments |
| 0.25% Trypsin | Cytiva | SH30042.01 | |
| 5 mL serological pipets | Celltreat | 229005B | |
| 6-well tissue culture plates | Celltreat | 229106 | |
| 10 cm cell culture dishes | Peak Serum | PS-4002 | |
| 10 ml serological pipets | Celltreat | 229210 | |
| 15 mL conical tubes | Celltreat | 667015B | |
| 50 mL conical tubes | Celltreat | 667050B | |
| BV2 microglia cell line | AcceGen Biotech | ABC-TC212S | |
| Cell lifter | Biologix Research Company | 70-2180 | |
| Crystal violet | Electron Microscopy Sciences | 12785 | |
| Dispase | Thermo Scientific | 17105041 | |
| DMEM/F12 | Caisson Labs | DFL14-500ML | |
| DNase-I | Sigma Aldrich | 11284932001 | |
| Essential amino acids | Thermo Scientific | 11130051 | |
| Ethanol (100%) | EMD Millipore | EX0276-1 | |
| Fetal bovine serum (heat inactivated) | Peak Serum | PS-FB4 | Can be purchased as heat inactivated or inactivated in the laboratory |
| Formaldehyde | EMD Millipore | 1.04003.1000 | |
| Glass 10 mL serological pipet | Corning | 7077-10N | |
| Hank’s Balances Salt Solution | Sigma Aldrich | H8264-500ML | |
| Hemocytometer/Neubauer Chamber | Daigger | HU-3100 | |
| High Glucose DMEM | Cytiva | SH30022.01 | |
| low glucose DMEM containing L-glutamine | Cytiva | SH30021.01 | |
| MEM/EBSS | Cytiva | SH30024.FS | |
| non-essential amino acids | Sigma-Aldrich | M7145-100M | |
| Paraformaldehyde (16%) | MP Biomedicals | 219998320 | |
| Penicillin/streptomycin/neomycin | Sigma-Aldrich | P4083-100ML | |
| Phosphate buffered saline | Cytiva | SH30256.01 | |
| Recombinant Mouse IFN-gamma Protein | R&D Systems | 485-MI | |
| Recombinant Mouse TNF-alpha (aa 80-235) Protein, CF | R&D Systems | 410-MT | |
| RNeasy mini kit | Qiagen | 74104 | |
| Sigmacote | Sigma Aldrich | SL2-100ML | Coat inside of glass pipets by aspirating up and down twice in Sigmacote and allowing to dry thoroughly. Wrap in aluminum foil and autoclave pipets 24 h later. |
| Stemxyme | Worthington Biochemical Corporation | LS004106 | Collagenase/Dispase mixture |
| Sterile, individually wrapped cotton swab | Puritan Medical | 25-8061WC | |
| Thincert Tissue Culture Inserts, 24 well, Pore Size=8 µm | Greiner Bio-One | 662638 | |
| Thincert Tissue Culture Inserts, 6 well, Pore Size=0.4 µm | Greiner Bio-One | 657641 |
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