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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Generation of Rat Intestinal 2D Monolayers from 3D Organoids

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Generation of Rat Intestinal 2D Monolayers from 3D Organoids

Generation of Rat Intestinal 2D Monolayers from 3D Organoids

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/200274-v

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02:47 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Rat Intestinal 2D Monolayers 3D Organoids EME Coating Collagen Coating Organoid Disruption Trypsin Treatment RIOM2D Y27632 2D Monolayers 3D Structures

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