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Chapters
- 00:00Title
- 01:43Introduction
- 02:22Use of High-Quality MISSION esiRNA Libraries for RNAi Screening
- 03:49Optimizing Transfection Prior to Screening
- 05:28Setting Up the Assay and Preparing for the Primary Screen
- 09:34The Primary Screen
- 12:03Secondary Screen of the Selected Genes for Hit Validation
- 13:40Representative Results Screening for Mammalian Cell Cycle Genes Using MISSION esiRNA
- 14:43Conclusion
Tags
MISSION EsiRNARNAi ScreeningMammalian CellsGene Expression ControlSmall Interfering RNAs (siRNAs)DicerRNA-induced Silencing Complex (RISC)Target MRNA CleavageLoss Of Gene Function StudiesChemical Synthesis Of SiRNAsEndonucleolytic CleavageTarget-specific Long Double-stranded RNAsRNase IIIEndoribonuclease-prepared SiRNAEfficacy Of SiRNAs And EsiRNAsOff-target Effects
