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自动疏水层析蛋白质纯化用柱选择
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
DOI:

10:21 min

September 21, 2011

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Chapters

  • 00:05Title
  • 01:30Buffer and Sample Preparation
  • 02:48Physical Setup and Plumbing of the DuoFlow Chromatography System
  • 04:11Priming System Lines,Programming Run Method, and Equilibrating HIC Columns
  • 05:43Sample Loading, Programming the Software, and Running the Column Scouting Protocol
  • 07:36Representative Chromatogram and Fluorescent Fraction Data
  • 09:23Conclusion

Summary

自动翻译

提出了一个自动化的方法,确定合适的疏水作用层析(HIC)在蛋白质纯化过程中使用的媒体。该方法利用一个中型高压液相色谱系统包括自动缓冲混合,动态样本循环注射,顺序列选择,多波长分析,并分割部分洗脱液收集。

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