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JoVE Journal Neuroscience
Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
 

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Article DOI: 10.3791/3466-v • 12:03 min • February 21st, 2012
February 21st, 2012 •
1, 2, 2, 1, 1, 3
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Center for Neural Development and Disease, University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

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Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

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Detection Microregional Hypoxia Mouse Cerebral Cortex Two-photon Imaging Endogenous NADH Fluorescence Oxygen Supply Blood Flow Tissue Oxygen Diffusion Visualized Experimental Protocol Methodological Protocol Perivascular Oxygen Gradients Non-linear Relationship Nicotinamide Adenine Dinucleotide (NADH) Fluorescence Intensity Oxygen Partial Pressure Two-photon Excitation Intrinsic NADH Tissue Fluorescence Texas-Red Dextran Advantages Standard Two-photon In Vivo Imaging Equipment Continuous Monitoring Depth Resolution Vulnerable Watershed Areas Decline In Vascular Oxygen Supply Microregional Cortical Oxygenation
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