Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Determining the Phagocytic Activity of Clinical…
JoVE Journal
Immunology and Infection

这篇 内容 是免费权限的.

JoVE Journal Immunology and Infection
Determining the Phagocytic Activity of Clinical Antibody Samples
 

Determining the Phagocytic Activity of Clinical Antibody Samples

Article DOI: 10.3791/3588-v • 09:06 min • November 30th, 2011
November 30th, 2011 •
1, 1, 1, 1, 1, 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

章节

总结概括
Automatic Translation

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

Tags

Phagocytic Activity Clinical Antibody Samples Fc Receptors Clearance Pathology Of Disease Variable Domains Innate Immune Cells Recombinant Monoclonal Antibody Therapy Natural Infection Vaccination IgG Subclass Glycosylation Functional Differences Antigen-specific Antibodies Disease State Susceptibility To Infection Progression Clinical Outcome Effector Function Enhance Infection Fc Receptor-driven Phagocytosis Immune Complexes Th1/Th2 Polarization Assay
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter