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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
 

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Article DOI: 10.3791/3791-v • 13:21 min • May 4th, 2012
May 4th, 2012 •
1, 1, 2, 1, 3, 4, 1
1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute

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In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.

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Chemically-blocked Antibody Microarray Multiplexed High-throughput Profiling Specific Protein Glycosylation Complex Samples Glycan Binding Protein Detection Post-translational Modification Physical Properties Of Proteins Chemical Properties Of Proteins Biological Properties Of Proteins Disease Progression Malignant Transformation Early Detection Biomarkers Cancer Biomarkers Practical Method Laboratory Settings Clinical Settings Chemically Blocked Antibody Microarray Glycan-binding Protein Detection Time Reduction Cost Reduction Lab Equipment Requirements
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