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JoVE Journal Bioengineering
Bakteriell Detection & Identification hjelp elektrokjemiske sensorer
 
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Bakteriell Detection & Identification hjelp elektrokjemiske sensorer

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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Vi beskriver en elektrokjemisk sensor assay metode for deteksjon og rask bakteriell identifikasjon. Analysen innebærer en sensor array funksjonaliseres med DNA oligonukleotide fangst prober for ribosomale RNA (rRNA) artsspesifikke sekvenser. Sandwich hybridisering av target rRNA med fangst sonde og en pepperrotperoksidase-linked DNA oligonukleotid detektor probe produserer en målbar amperometrisk strøm.

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Bioteknologi mikrobiologi genetikk molekylærbiologi cellebiologi biokjemi Biomedical Engineering medisin immunologi Bakterier Elektrokjemisk sensor ribosomal RNA rRNA 16S RNA DNA sonde analysen
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