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Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
 

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

Article DOI: 10.3791/50373-v • 13:38 min • May 10th, 2013
May 10th, 2013 •
1, 1, 1, 2, 3, 2, 2, 1
1Biology Department, Western Washington University, 2Washington State University Northwestern Research and Extension Center, 3Department of Plant and Soil Science, Texas Tech University

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Plastic films labeled "biodegradable" are commercially available for agricultural use as mulches. Tillage represents an attractive disposal method, but degradation under field conditions is poorly understood. The purpose of this study was to develop methods for isolating native soil fungi and bacteria that colonize plastic mulch films after field burial.

Tags

Isolation Native Soil Microorganisms Biodegradable Plastic Mulch Films Agriculture Fungi Degradation Plastics Agar-based Media Visualization In Situ Degradation Soil Matrix Pure Polymers Additives Microbial Growth Procedures Fungal Isolates Growth Carbon Source Scanning Electron Microscopy Identification Ribosomal RNA Gene Sequences Bacteria Isolation
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