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G蛋白偶联受体的基于荧光的钙动员含量表征
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
DOI:

11:49 min

July 28, 2014

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Chapters

  • 00:05Title
  • 02:10Transient Transfection of HEK293T Cells
  • 04:08Calcium Mobilization Assay
  • 08:17Assay on the Station Device
  • 09:29Results: Concentration Dependent Activation of the Drosophila Short Neuropeptide F Receptor by sNPF Peptides
  • 10:59Conclusion

Summary

自动翻译

在这里所描述的基于荧光的钙动员测定法是孤儿G蛋白偶联受体(GPCRs)的功能性活化配位体(多个)的识别介质通量相反的药理筛选系统。

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