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Elektrospinproces Growth Factor Releasing microsferen in Stapel- Steigers
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JoVE 杂志 生物工程
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
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Elektrospinproces Growth Factor Releasing microsferen in Stapel- Steigers

DOI:
10.3791/51517-v

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09:29 min

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August 16, 2014

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,
1Biomedical Engineering,Wayne State University

Chapters

  • 00:05Title
  • 01:00Water/Oil/Water Double Emulsion Microsphere Production
  • 03:52Electrospinning with Microspheres
  • 05:28Protein Bioactivity Testing
  • 07:41Results: Microspheres within Scaffold Release NGF That Promotes Primary Nerve Cell Growth
  • 09:09Conclusion

Summary

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This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.

Tags

Keywords: ElectrospinningGrowth FactorMicrospheresFibrous ScaffoldNerve Cell GrowthMechanical PropertiesTopographical CuesAdhesionChemical SignalsHyaluronic AcidPoly(lactic-co-glycolic Acid)PLGANerve Growth FactorNGFProtein ViabilityExtended Release

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