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High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
 

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Article DOI: 10.3791/52242-v • 09:19 min • January 4th, 2015
January 4th, 2015 •
1, 1,3, 2,3, 2, 2, 1, 2,3
1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Genetics, Development and Cell Biology, Iowa State University, 3Biology Program, Iowa State University

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This study describes an experimental platform to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.

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High Throughput Characterization Adult Stem Cells Engineered Delivery Therapeutic Factors Neuroprotective Strategies Mesenchymal Stem Cells Bone Marrow Cellular Resource Diseases Genetically Modified Lentiviral Vectors Brain-derived Neurotrophic Factor (BDNF) Glial Cell-derived Neurotrophic Factor (GDNF) Green Fluorescent Protein (GFP) In Vivo Transplant Studies Cell Behavior Culture Substrates Cell Adhesion Proliferation Survival Cell Migration Four Subpopulations Of Engineered MSCs High Content Screening (HCS) Image Analysis Poly-L-lysine Fibronectin Collagen Type I Laminin Entactin-collagen IV-laminin (ECL) Ki67 Immunolabeling Cell Proliferation Propidium Iodide Staining Cell Viability
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