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Chapters
- 00:05Title
- 01:54Cholesterol Depletion of J774A.1 Cells with MβCD
- 03:26Infection of Macrophages with C. neoformans H99
- 08:20Results: C. neoformans Infection of Cholesterol-depleted Cells
- 10:02Conclusion
In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.
