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Chapters
- 00:05Title
- 02:01Bacteria Preparation
- 02:55Fly Infection by Nanoinjector
- 04:49Fly Injection by Septic Pinprick
- 05:49Assaying Bacterial Load Post-infection
- 08:02Results: Bacterial Infection of Drosophila melanogaster
- 09:15Conclusion
Drosophila melanogaster is an outstanding model organism for studying innate immune systems and the physiological consequences of infection and disease. This protocol describes how to deliver robust and quantitatively repeatable bacterial infections to D. melanogaster, and how to subsequently measure infection severity and quantify the host immune response.
