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1Neurodegenerative Diseases Unit,ANSES – French Agency for Food, Environmental and Occupational Health & Safety, 2Indicia Production, 3AJ Roboscreen GmbH, 4Epidemiology Unit,ANSES – French Agency for Food, Environmental and Occupational Health & Safety, 5Experimental Facilities,ANSES – French Agency for Food, Environmental and Occupational Health & Safety
Chapters
- 00:05Title
- 02:32Dissect Brain Regions
- 04:53Prepare Brain Homogenates
- 06:02Detection of αSD by ELISA
- 08:49Results: Disease Detection Using Enhanced ELISA
- 10:39Conclusion
An ELISA offering a novel quantitative approach is described. It specifically detects disease-associated α-synuclein (αSD) in a transgenic mouse model (M83) of synucleinopathy using several antibodies against either the Ser129 phosphorylated αS form or the C-terminal part of the protein.
