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Isolamento e cultura primária de células de camundongo aórtica endoteliais
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生物学
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JoVE 杂志 生物学
Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Isolamento e cultura primária de células de camundongo aórtica endoteliais

DOI:
10.3791/52965-v

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08:20 min

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December 19, 2016

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, ,
1Center for Molecular Medicine and Genetics,Wayne State University, 2Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine,Central South University

Chapters

  • 00:05Title
  • 00:54Preparing Aortic Segments
  • 03:14Seeding Aortic Segments on a Matrix
  • 04:48Passaging the Endothelial Cells
  • 06:41Aorta-derived Endothelial Cell Characterization
  • 07:48Conclusion

Summary

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The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

Tags

Keywords: Mouse Aortic Endothelial CellsPrimary CultureIsolationAortaEndotheliumHeparinPBSEndothelial Growth MediumMatrixSix-well Plate

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