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Journal / Developmental Biology / Analysis of Zebrafish Larvae Skeletal Muscle…
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Developmental Biology

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JoVE Journal Developmental Biology
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
 

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Article DOI: 10.3791/53183-v • 07:34 min • November 30th, 2015
November 30th, 2015 •
*1,2, *3,4, 1,2, 1,2,4
1Program in Genetics & Genome Biology, The Hospital for Sick Children, 2Department of Molecular Genetics, The University of Toronto, 3Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 4Departments of Pediatrics and Neurology, University of Michigan
* These authors contributed equally

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In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.

Tags

Zebrafish Larvae Skeletal Muscle Integrity Evans Blue Dye Neuromuscular Disorders Muscle Membrane Degenerating Muscle Fibers Abnormal Membrane Integrity Muscular Dystrophies Cell Permeable Dye EBD Injection Muscle Dissection EBD Uptake Live Zebrafish Co-injection Strategy EBD Analysis Zebrafish Models Of Neuromuscular Disease
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