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Chapters
- 00:05Title
- 00:45Density Gradient Centrifugation
- 01:50Collagen Coating of T-75 Flask and Preparation of Culture Medium
- 03:00Collection and Plating of Peripheral Blood Mononuclear Cells
- 04:51Long-term Cell Culture
- 06:58Freezing and Thawing BOEC Cultures
- 08:44Results: Brightfield Images of BOEC Cultures and Immunofluorescent Staining of BOECs
- 09:47Conclusion
This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.
