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JoVE Journal Neuroscience
Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
 

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

Article DOI: 10.3791/53743-v • 14:28 min • March 16th, 2016
March 16th, 2016 •
1, 2, 1,2
1Curriculum in Neurobiology, The University of North Carolina at Chapel Hill, 2Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill

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Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.

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Combined Methodologies Plasma Membrane Delivery Axon Branching Neuronal Morphogenesis Quantifiable Observations Temporal And Spatial Occurrence Exocytic Vesicle Fusion Neuronal Development Live Cell Imaging High Spatial Resolution High Temporal Resolution Biochemical Approaches Population-based Information Culturing And Simulating Cortical Neurons Netrin-1 Control Homogenization Buffer Protein Concentrations Triton X-100 Triturate Mixture Solubilize Proteins Centrifuge
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