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JoVE Journal Medicine
Cutaneous Surgical Denervation
 

Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease

Article DOI: 10.3791/54050-v • 08:01 min • June 26th, 2016
June 26th, 2016 •
1, *2, *1
1Dermatology, Cell and Developmental Biology, University of Michigan, 2Dermatology Branch, National Cancer Institute, National Institutes of Health
* These authors contributed equally

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This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.

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Cutaneous Surgical Denervation Nerves Mouse Models Skin Disease Homeostasis Pathological Skin Intact Skin Denervated Skin Visual Demonstration Sedation Warming Pad Aseptic Surgical Area Electric Clipper Dorsal Side Biopsy Site Longitudinal Sections Hair Follicles Scalpel Excision Epidermis Dermis
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