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Chapters
- 00:05Title
- 00:51Thymus Digestion
- 03:50Thymic Epithelial Cell (TEC) Enrichment
- 04:58TEC/EAK Aggregate Generation
- 06:52Results: Representative TEC Enrichment and Aggregation
- 07:26Conclusion
This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.
