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Los métodos para la determinación de las tasas de glucosa y la oxidación de ácidos grasos en el corazón de rata aislado de Trabajo
JoVE 杂志
生物学
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JoVE 杂志 生物学
Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
DOI:

12:24 min

September 28, 2016

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Chapters

  • 00:05Title
  • 01:07Rat Heart Isolation and Cannulation
  • 04:51Measurement of Cardiac Function and Sample Collection
  • 07:17Determination of Myocardial Glucose Oxidation Rate
  • 08:48Determination of Myocardial Oleate Oxidation Rates
  • 10:14Results: Measurements from Two Stressed Hearts
  • 11:40Conclusion

Summary

自动翻译

The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.

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