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Chapters
- 00:05Title
- 01:08Transduce Donor Bone Marrow Cells
- 04:56Transplant Donor Cells into Recipient Mice
- 06:43Results: Reconstitution of Hematopoietic Cells in Mice Using Transduction-Transplantation
- 07:32Conclusion
This manuscript provides a description of the methodology used to establish transduction-transplantation mouse models. A detailed account is given of technical errors to avoid when performing bone marrow transplants. A clear understanding should be gained of the importance of high viral titer, transfection/transduction, and irradiation.
