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Cancer Research

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JoVE Journal Cancer Research
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
 

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Article DOI: 10.3791/54633-v • 09:13 min • October 24th, 2016
October 24th, 2016 •
1, 1, 2, 1,3, 1, 2, 1
1Department of Applied Biochemistry, Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology, Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering, Institute of Biotechnology, Berlin University of Technology

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The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.

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Viral Vectors Three-dimensional Liver Model Human Hepatocellular Carcinoma Cell Line HepG2 Gene Transfer Animal Welfare Animal Experiments Liver Scaffolds Hepatic Cell Line Hep G2 Medium Fetal Calf Serum Gulatmine Penicillin Streptomycin Trypsin Solution Exposure
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