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Immunology and Infection

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JoVE Journal Immunology and Infection
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
 

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

章节

总结概括
Automatic Translation

Herein we describe a novel method to generate antigen-specific T cell receptors (TCRs) by pairing the TCRα or TCRβ of an existing TCR, possessing the antigen-specificity of interest, with complementary hemichain of the peripheral T cell receptor repertoire. The de novo generated TCRs retain antigen-specificity with varying affinity.

Tags

De Novo Antigen-specific T Cell Receptors Retroviral Transduction Centric Hemichain Gene Engineered Immunotherapy PCR Product Clone Assembly Master Mixery Agent Chemically Confident E Coli Lysogeny Broth Medium Midiprep Kit Plasmin Purification 293GPG Cell Transfection
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