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Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique
 

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Article DOI: 10.3791/54998-v • 13:19 min • December 16th, 2017
December 16th, 2017 •
*1, *1, 1, 1, 2, 1,3, 1, 1,4, 1
1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, 2Department of Anesthesiology and Critical Care Medicine, Medical University Innsbruck, 4Department of Cardiac Surgery, Medical University Innsbruck, 3Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine
* These authors contributed equally

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总结概括
Automatic Translation

Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.

Tags

Mouse Model Pancreas Transplantation Modified Cuff Technique Anesthesia Ketamine Xylazine Abdominal Region Recipient Operative Field Chlorhexidine Laparotomy Pubic Region Xiphoid Viscera Heparin Infrarenal Vena Cava Abdominal Aorta Left Renal Vein Liver Hilum Common Bile Duct Portal Vein Venous Anastomosis
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