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Canlı Görüntüleme taksan dirençli Meme Kanserlerinde Mikrotubul dinamik dengesizlik Eğitim için
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
DOI:

06:02 min

February 20, 2017

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Chapters

  • 00:05Title
  • 00:37Cell Culture and Seeding
  • 01:43GFP-tagged α-tubulin Expression
  • 03:34Image Processing and Data Analysis
  • 04:27Results: Representative Effects of Docetaxel in the Microtubule Dynamics of MCF-7TXT and MCF-7CC Cells
  • 05:05Conclusion

Summary

自动翻译

In this paper, we report a protocol describing an in vivo method to measure microtubule dynamic instability in docetaxel-resistant breast cancer cells (MCF-7TXT). In this method, a deconvolution microscopy imaging system is used to detect the expression of GFP-tubulin in target cells.

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