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JoVE Journal Developmental Biology
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
 

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Article DOI: 10.3791/55120-v • 09:07 min • February 24th, 2017
February 24th, 2017 •
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1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine

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Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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Rapid Isolation BMPR-IB+ Adipose-derived Stromal Cells Calvarial Defect Healing Model Osteogenic Activity Craniofacial Regenerative Biology Adipose-derived Stem Cells Fully Functional Bone Tissue Damaged Bone Defects Fresh Human Tissue Cell Culture Gene Expression Cell Service Marker Expression Large Bone Defects Cancer Trauma Infection Regenerative Fashion Visual Demonstration Calvarial Defect Creation Domed Skull Dura Mater Abdominal Fat Collection Flank Fat Collection Thigh Subcutaneous Fat Collection Sterile PBS
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