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Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
 

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D

Article DOI: 10.3791/55304-v • 11:27 min • March 16th, 2017
March 16th, 2017 •
*1, *1, 1, 1, 1, 1,2, 1,2, 1,2, 1
1Department of Medicine, University of Illinois at Chicago, 2Department of Research, Jesse Brown VA Medical Center
* These authors contributed equally

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We describe simple methods to study the regulation of intestinal serotonin transporter (SERT) function and expression using an in vitro cell culture model of Caco-2 cells grown in 3D and an ex vivo model of mouse intestines. These methods are applicable to the study of other epithelial transporters.

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Epithelial Transport Protein Function Expression Native Intestine Caco-2 Cells 3D Culture Ussing Chamber Serotonin Transporter Regulation Cell-to-cell Interactions Cell-to-extracellular Matrix Interactions Monolayers Transport Function Measurements Therapeutics Discovery Electrolyte Transporters Sodium Transporters Chloride Transporters Seromuscular Layer Stripping Intestinal Mucosa Mounting
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