Journal
/
/
非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
DOI:

11:55 min

April 03, 2017

,

Chapters

  • 00:05Title
  • 00:43Pre-embedding Immunohistochemistry
  • 03:11Osmification and Embedding for Electron Microscopy Observation
  • 06:23Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
  • 09:05Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
  • 11:08Conclusion

Summary

自动翻译

在这里,我们提供了一种简单,低成本,和时间高效的协议进行化学固定与丙烯醛固定液灵长类脑组织,从而允许长期保存,其与预嵌入-免疫组化透射电子显微镜兼容。

Related Videos

Read Article