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JoVE Journal Biochemistry
Måling av G-proteinkoblet reseptor-signalering via radiomerket GTP-binding
 
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Måling av G-proteinkoblet reseptor-signalering via radiomerket GTP-binding

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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Guanosintrifosfat (GTP) binding er en av de tidligste hendelsene i G-protein-koblet reseptor (GPCR) aktivering. Denne protokollen beskriver hvordan man farmakologisk karakteriserer spesifikke GPCR-ligand-interaksjoner ved å overvåke bindingen av den radiomerkede GTP-analogen, [35S] guanosin-5'-O- (3-tio) trifosfat ([35S] GTPγS), i Svar på en ligand av interesse.

Tags

Biochemistry Cellular fraksjonering membranpreparasjon GPCR agonist antagonist GTPγS-binding farmakologi μ-opioidreseptor
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