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JoVE Journal Neuroscience
Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space
 

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

Article DOI: 10.3791/55755-v • 10:45 min • July 24th, 2017
July 24th, 2017 •
*1, *2,3, 4, 5, 5, 2
1Department of Medicine, University of Virginia, 2Department of Cell Biology, SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, 4Division of Neonatology, University of Virginia, 5Department of Neuroscience and Physiology, New York University School of Medicine
* These authors contributed equally

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This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.

Tags

Real-time Iontophoresis Tetramethylammonium Volume Fraction Tortuosity Brain Extracellular Space Characterization Physiology Disease Iontophoretic Microelectrode Troubleshooting Ion-selective Microelectrode Recording ACSF Submersion Chamber Temperature Controller Ground Electrode Porous Cup Agarose
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