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Immunology and Infection

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JoVE Journal Immunology and Infection
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
 

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Article DOI: 10.3791/55760-v • 07:58 min • May 30th, 2017
May 30th, 2017 •
*1, *1, 1, 1, 1
1Department of Biological Sciences, Plymouth State University
* These authors contributed equally

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This manuscript describes proper Vibrio cholerae maintenance techniques in addition to a series of biochemical assays, collectively utilized for quick and reliable differentiation between clinical and environmental V. cholerae biotypes in a laboratory setting.

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Laboratory Techniques Maintain Differentiate Biotypes Vibrio Cholerae Clinical Isolates Environmental Isolates Protocols Investigator Genotypic Characteristics Phenotypic Characteristics Clinical Relevance Advantage Assays Systematic Approach Alternative Labor Intensive Experiments Biochemical Capabilities Metabolic Capabilities Enteric Bacteria Time Management Incubation Times Optimal Results Biochemical Assays Visual Demonstrations Spotting Protocols Stabbing Protocols Splatter Cross-contamination
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