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JoVE Journal Bioengineering
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
 

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Article DOI: 10.3791/55873-v • 09:19 min • September 15th, 2017
September 15th, 2017 •
1,2,3, 1,2, 4, 4, 1,2,3
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 3Department of Bioengineering, Northeastern University, 4Department of Bioengineering, University of Texas at Arlington

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In this method, we use photopolymerization and click chemistry techniques to create protein or peptide patterns on the surface of polyethylene glycol (PEG) hydrogels, providing immobilized bioactive signals for the study of cellular responses in vitro.

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Patterning Bioactive Proteins Peptides Hydrogel Photochemistry Biological Applications Immobilized Bioactive Spatial Protein Patterns Cellular Responses In-vivo Signals Biomaterial Strategies Substrate Stiffness Protein Patterning Tissue Engineering Regenerative Medicine Technologies Tissue Development Stem Cell Fate Polyethylene Glycol Diacrylate Photo Initiator Lithium Phenyl-2 4 6-trimethylbenzoylphosphinate ECM Protein Fibronectin
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