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人胚胎干细胞源性视网膜色素上皮细胞移植视网膜大眼模型的地理萎缩
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生物学
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JoVE 杂志 生物学
Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
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人胚胎干细胞源性视网膜色素上皮细胞移植视网膜大眼模型的地理萎缩

DOI:
10.3791/56702-v

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11:03 min

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January 22, 2018

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, , , , ,
1Clinical Neuroscience, Section for Ophtalmology and Vision,Karolinska Institutet, 2Clinical Sciences, Intervention and Technology, Division of Obstetrics and Gynecology,Karolinska Institutet

Chapters

  • 00:05Title
  • 01:12Subretinal Injection of Sodium Iodate into a Large-eyed Animal Model
  • 04:36Subretinal Transplantation of hESC-RPE Cells in Treated Animals
  • 07:35In Vivo Retinal and Subretinal Imaging
  • 08:47Results: Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells
  • 10:22Conclusion

Summary

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视网膜色素上皮细胞可作为晚期相关黄斑变性的细胞替代疗法。该协议描述了大眼睛的地理萎缩模型的产生和人类胚胎干细胞源性视网膜色素上皮细胞视网膜移植到这种疾病模型。

Tags

Subretinal TransplantationHuman Embryonic Stem CellRetinal Pigment Epithelial CellsLarge-eyed Animal ModelGeographic AtrophyRegenerative MedicineAge-related Macular DegenerationOptical Coherence TomographyMicrosurgeryPars Plana TechniqueVitrectomySubretinal Injection

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