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Journal / Developmental Biology / Combinational Treatment of Trichostatin A and…
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Developmental Biology

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JoVE Journal Developmental Biology
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
 

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Article DOI: 10.3791/57036-v • 09:52 min • April 26th, 2018
April 26th, 2018 •
1, 2, 3, 4, 1,5
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University

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We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse cloning.

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Trichostatin A Vitamin C Cloning Mice Somatic Cell Nuclear Transfer Production Efficiency Reconstructed Embryos Basic Biology Developmental Biology Endangered Animal Field Embryonic Development Nuclear Programming Genetic Research Conservation Culture Medium BSA Solution Ion Exchange Resin Beads Sodium Bicarbonate DBSA Stock Solution Superovulation Dissection Techniques
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