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JoVE Journal Cancer Research
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia
 

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Article DOI: 10.3791/57787-v • 09:02 min • November 26th, 2018
November 26th, 2018 •
*1, *2,3, 1, 4, 5, 6, 7, 2,3, 1,2, 8
1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 2Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 3Department of Molecular Medicine and Surgery, Karolinska Institutet, 4Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, 5Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, 6Hematology Department, Nikea General Hospital, 7Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, 8Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele
* These authors contributed equally

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Herein, we present a protocol that details the technical aspects and essential requirements to ensure robust IG gene sequence analysis in patients with chronic lymphocytic leukemia (CLL), based on the accumulated experience of the European Research initiative on CLL (ERIC).

Tags

Immunoglobulin Gene Sequence Analysis Chronic Lymphocytic Leukemia Patient Material Sequence Interpretation Prognosis Technique Simple Fast Accurate Implications Robust Prognostication Risk Certifications Therapeutic Management Visual Demonstration PCR Product Selection Digest Sequence Analysis Precision Experience Procedure EDTA Sterile Collection Tube Peripheral Blood RPMI Medium Pipette Density Gradient Medium Centrifuge Mononuclear Cell Ring
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