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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
 

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

Article DOI: 10.3791/59072-v • 06:32 min • July 2nd, 2019
July 2nd, 2019 •
1, 1, 1
1Department of Pharmacology and Toxicology, University of Mississippi Medical Center

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The primary goal of this article is to provide details of how to record membrane potential (Vm) from the middle cerebral artery using the microelectrode impalement method. The cannulated middle cerebral artery is equilibrated to gain myogenic tone, and the vessel wall is impaled using high resistance microelectrodes.

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Microelectrode Impalement Method Record Membrane Potential Cannulated Middle Cerebral Artery Measure Membrane Potential Normal Blood Vessels Diseased Blood Vessels Evaluate Pharmacological Agents Modulate Membrane Potential Vascular Tone Blood Flow Physiological Range Vascular Smooth Muscles Syncytium Dual Channel Differential Electrometer Amplifier BNC-BNC Cable Digitizer Micromanipulator Microscope Myograph Vibration-free Table Borosilicate Glass Microelectrodes Standard Puller Taper Diameter Microfiber Syringe Potassium Chloride
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