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Developmental Biology

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JoVE Journal Developmental Biology
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
 

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Article DOI: 10.3791/59533-v • 08:16 min • June 6th, 2019
June 6th, 2019 •
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1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

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Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.

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Isotropic Light-Sheet Microscopy Automated Cell Lineage Analyses Caenorhabditis Elegans Embryogenesis Subcellular Resolution Cell Morphologies Axons Dendrites Confocal Microscopy Dual-view Inverted Selective Plane Illumination Microscopy DiSPIM Methylcellulose Solution Nematode Growth Medium Plate M9 Buffer Microcapillary Pipette Steel Imaging Chamber Sample Holder
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