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Genetics

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JoVE Journal Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
 

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Automatic Translation

Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.

Tags

Axolotl Gene Knock-out Spinal Cord Neural Stem Cells Electroporation CAS9 Protein-gRNA Complexes Molecular Mechanisms Regeneration Gene Function Developmental Effects Cell Types Spinal Cord Injury Injection Capillary Cas9 Guide RNA Ribonuclear Protein Mixture Cas9 Buffer Nuclease-free Water Minus 80 Degrees Celsius Agarose Electropor
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